An Embroidered Pin Cushion in late 16thC style by Genevieve d'Aquitaine, Baroness of the Court of Atlantia, Mistress of the Order of the Pelican

described at http://genvieve.net/sca/embroidery/pincushion.html

She used Isabella, Redde, Carnacion, Purple, Indico Blewe, Graie Blewe, Bisse and Black

This lady has also made notes on the individual motifs used on the Plimouth Jacket, including the colours of GST and silk perle, and the stitches used for each one :

http://genvieve.net/sca/embroidery/index.html

Her LiveJournal "PinkLeader's Pontifications" http://pinkleader.livejournal.com/tag/plimoth talks extensively about her participation in the Plimouth Project.

I found a photo of a detached butterfly wing that she made, which I'm quite excited about, since we haven't seen these on the Plimouth site (although this is her original piece, not part of the Jacket)

http://flickr.com/photos/jenthies/3178307539/in/set-72157594385362637/

and the talk of silk "heathering" - combination of colours into one thread, at http://pinkleader.livejournal.com/113854.html#cutid1 is most interesting.

I do know of an article that could be relevant here "MakingPerleCoton" - http://www.lkreinmiller.com/heartwork/Tech/perle/MakingPerleCoton.pdf

When I've used two colours in my sampler, I've just used two differently coloured threads in my needle!